Monday, 5 May 2014

Basic principles of r-DNA technology

The basic principles of recombinant DNA technology are reasonably simple, and broadly involve the following stages.

1. Generation of DNA fragments and selection of the desired 
    piece of DNA (e.g.  a human gene).

2. Insertion of the selected DNA into a cloning vector
    (e.g.  a plasmid) to create a recombinant DNA.

3. Introduction of the recombinant vectors into host cells
    (e.g.  bacteria).

4. Multiplication and selection of clones containing the 
    recombinant molecules.

5. Expression of the gene to produce the desired product.

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